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Il y a 3 jours · In 1985, Walker et al. first demonstrated that the addition of double-stranded DNA (dsDNA) to multiple cell extracts, mimicked DNA double-stranded breaks (DSB) and triggered the phosphorylation of ...
Il y a 4 jours · We therefore developed click editors (CEs; Fig. 1a), which leverage these favorable enzymatic and template properties to install genome edits without requiring DNA double-stranded breaks.
Il y a 2 jours · POLQ is reported to be involved in the microhomology-mediated end-joining repair of double-stranded DNA breaks. 40 Several studies have indicated that POLQ is highly expressed in human cancers, 41, 42 and its overexpression is linked to poor survival outcomes in breast cancer. 43 Currently, there are few reports regarding the role of POLQ in cancer.
Il y a 3 jours · Ubiquitylation is critically implicated in the recognition and repair of DNA double-strand breaks. The adaptor protein MDC1 mediates the recruitment of the key DNA damage responsive E3 ubiquitin ligase RNF8 to the break sites.
Il y a 2 jours · Mutations of OsALKBH5 cause reduced double-strand break (DSB) formation, severe defects in DSB repair, and delayed meiotic progression, leading to complete male sterility. Transcriptome analysis and m 6 A profiling indicate that OsALKBH5-mediated m 6 A demethylation stabilizes the mRNA level of multiple meiotic genes directly or indirectly, including several genes that regulate DSB formation ...
Il y a 4 jours · Properly spaced single-stranded breaks in the host DNA can trigger homology directed repair, which is less error-prone than the non-homologous end joining that typically follows a double-stranded break.
Il y a 4 jours · To characterize how DdrC interacts with double-strand breaks, we assessed whether DdrC has a preference for overhang type at the terminal ends of linear DNA. In this assay, one ds-break was introduced into pUC19 plasmid harboring either 0, 2 or 4 nt overhangs (5′) using SmaI, NdeI and SalI endonucleases, respectively.
